E3 Journal of Environmental Research and Management

E3 Journal of Environmental Research and Management Vol. 10 (1) pp. 005-011, August 2019; © E3 Journals; ISSN 2141-7466

Protective effect of methanolic extract of laportea aestuans on indomethacin-induced kidney damage in male wistar rats

Abstract

This study aimed at evaluating the nephroprotective role of different doses of the methanol extract of Laportea aestruans leaves on indomethacin-induced nephrotoxicity in adult male Wistar rats. Forty rats were divided into five groups of eight rats and pretreated orally for three days with methanol extract of Laportea aestruans before administration of indomethacin. Rats in group one; normal control received 0.5 ml normal saline (0.9 % v /v). Rats in group two received 20 mg/kg body weight indomethacin alone. Rats in group three, four and five received 200, 400 and 800 mg/kg body weight of methanol extract of Laportea aestruans. At the expiration of the experiment, rats were sacrificed, blood and the kidney were collected for renal functions tests. The results showed that administration of indomethacin alone caused a significant (p ˂ 0.05) increase in the concentrations of creatinine, urea, uric acid, cholesterol and total protein and the activity of glucose-6-phosphatase and renal 5΄-nucleotidase in the piroxicam alone treated group. However, pretreatment methanol extract of Laportea aestruans showed a dose-dependent decrease in the concentrations and the activities of all the kidney markers assayed for. The results show that methanol extract of Laportea aestruans possesses nephroprotective potential against indomethacin-induced renal damage.

Keywords: Nephroprotective, Laportea aestruans, indomethacin