A review of cyclostationary feature detection based spectrum sensing technique in cognitive radio networks; Effect of quality work life relationship on occupational stresses among Bu-alisina university staff in Hamadan province, IR Iran; Partial purification of α-amylase expressed by a tropical mutant strain of Aspergillus flavus IFE 03.

E3 Journal of Scientific Research

E3 Journal of Scientific Research Vol. 4 (3) pp. 052-055, September 2016; © E3 Journals; ISSN 2276-9919
DOI: http://dx.doi.org/10.18685/EJSR(4)3_EJSR-16-023

Partial purification of α-amylase expressed by a tropical mutant strain of Aspergillus flavus IFE 03

Adejuwon AO1 * , Olutiola PO3 , Segal SJ4
1 Department of Microbiology, Faculty of Sciences, Lead City University, Ibadan, Nigeria
3 Department of Biological Sciences, Bowen University, Iwo, Nigeria
4 Weill Medical College, Cornell University, Manhattan, New York, United States of America
*Corresponding Author E-mail: ao_adejuwon@yahoo.ca
Accepted 14 September 2016

Abstract

A tropical mutant strain of Aspergillus flavus induced by ultraviolet radiation of a tropical wild type (Aspergillus flavus IFE 03) expressed α-amylase activity in a defined medium with starch as carbon source and peptone as nitrogen source of growth. The enzyme was subjected to partial purification by ammonium sulphate precipitation followed by dialysis. Specific activity of the enzyme increased 6.58 fold while recovery was 32.2% after dialysis.

Keywords: Aspergillus flavus, partial purification, α-amylase, tropical, mutant strain

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