E3 Journal of Agricultural Research and Development
E3 Journal of Agricultural Research and Development Vol. 3 (6) pp. 094-097, August 2013; © E3 Journals; ISSN 2276-9897
Genetic diversity among omani basil (ocimum basilicum l.) landraces using RAPD markers
AHMED YAHYA AL-MASKRI 1 * , MUHAMMAD MUMTAZ KHAN 1 , SULTAN HABIBULLAH KHAN 11 Department of Crop Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Sultanate of Oman
2 Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan
*Corresponding Author E-mail: sultan@uaf.edu.pk
Accepted 27 August 2013
Abstract
Basil (Ocimum L.), a medicinal and aromatic plant genus, is widely used in food and pharmaceutical industry for ages. Our previous studies reported high potential of essential oils in the unexplored and un-exploited landraces of Omani basil. In the present study, nine Omani landraces of common basil (Ocimum basilicum L.) were analyzed for the assessment of genetic diversity with RAPD markers. Out of fifteen tested seven primers generated a total of 25 alleles with an average of 3.57 per locus. Maximum six alleles were amplified with the primer OPK16 while primers OPK1 and OPK10 amplified only two alleles. Mean polymorphic information content (PIC) was 0.5. Dendrogram based on similarity matrix grouped landraces 1 and 3; 4and W together showing similarity coefficient of 1.
Keywords: Landraces, Basil, Genetic Diversity
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